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The samples were opened to expose the bone marrow cavity usi

 
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parent3cell
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PostPosted: Mon Jan 22, 2018 7:55 am    Post subject: The samples were opened to expose the bone marrow cavity usi Reply with quote

They were then dehydrated in graded ethanol, defatted in xylene, and embedded undecalcified in methyl methacrylate. Frontal sections were cut at thicknesses of 4-and 10-mm. The 4-mm sections were stained by Goldner’s Trichrome for static histomorphometric measurements. The 10- mm unstained sections were used for dynamic histomorphometric analyses. A digitizing image Fulvestrant|ICI 182780 Estrogen Receptor inhibitor analysis system was used for quantitative bone histomorphometric measurements. Briefly, the regional of interest were the proximal tibial growth plate and the proximal tibial metaphysis located between 1 and 4 mm distal to the growth plate-epiphyseal junction. Static measurements included total tissue volume, trabecular bone volume, marrow fatty area, trabecular bone surface, osteoclast surface and osteoblast surface. Dynamic measurements include interlabel width in the growth plate of PTM, trabecular single-labeled GANT61|NSC 136476 Hedgehog inhibitor surface, double labeled surface and interlabel width, and endocortical single-labeled surface, double labeled surface and endocortical interlabel width. These parameters were used to calculate longitudinal growth rate, percentages of trabecular bone volume, trabecular number, trabecular thickness, trabecular separation, marrow fatty area, osteoclast surface, osteoblast surface,
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